determination of t-2 mycotoxin fusarium strains by hplc with fluorescence detector

نویسندگان

reza kachuei

sassan rezaie

mohammad hossein yadegari

naser safaie

چکیده

t-2 toxin is the most poisonous trichothecene produced by fusarium species especially f.sporotrichioides . t-2 toxin is a biological contaminant in a number of agricultural commodities that can cause severe diseases among humans and animals and even lead to death. the aim of the current study is the analysis of t-2 mycotoxin in fusarium species by high-performance liquid chromatography (hplc) combined with florescence detection and derivatization with 1-antroylnitrile (1-an). totally, 11 fusarium isolates and reference strains were studied. the isolates were tested for the t-2 toxin production after growing on rice substrate followed by using specific “multisep 225 trich clean up columns” purification. in this study, t-2 toxin production was ranged from 197.05ug/kg to 8503.07ug/kg. this is the first study of t-2 toxin analysis by hplc-f in iran.                                         keywords fusarium ,trichothecene , t-2 toxin , hplc-f

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applied biotechnology reports

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